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e-64d (cat. no. e8640)  (Millipore)


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    Millipore e-64d (cat. no. e8640)
    E 64d (Cat. No. E8640), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e-64d+(cat+no+e8640)/e64d/pmc09310534__jciinsight___7___152783___s132-141-11-29
    Average 90 stars, based on 1 article reviews
    e-64d (cat. no. e8640) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Supplementary Information for FAM114A1 Influences Cardiac Pathological Remodeling by Regulating Angiotensin II Signaling
    Article Snippet: Reagents, antibodies, plasmids, and siRNAs Human angiotensin II (Cat. No. A9525), E-64d (Cat. No. E8640), 2,3 Butanedione Monoxime (Cat. No. B0753), and 5-Bromo-2'-deoxyuridine (Cat. No. 10280879001) were purchased from Sigma Aldrich, USA.

    Article Title: Mixed-Charge Nanocarriers Allow for Selective Targeting of Mitochondria by Otherwise Nonselective Dyes.
    Article Snippet: Targeted delivery of molecular cargos to specific organelles is of paramount importance for developing precise and effective therapeutics and imaging probes.. This work describes a disulfide-based delivery method in which mixedcharged nanoparticles traveling through the endolysosomal tract deliver noncovalently bound dye molecules selectively into mitochondria.. This system comprises three elements: (1) The nanoparticles deliver their payloads by a kiss-and-go mechanism that is, they drop off their dye cargos proximate to mitochondria but do not localize therein; (2) the dye molecules are by themselves nonspecific to any cellular structures but become so with the help of mixed-charge nanocarriers; and (3) the dye is engineered in such a way as to remain in mitochondria for a long time, up to days, allowing for observing dynamic remodeling of mitochondrial networks and long-term tracking of mitochondria even in dividing cells.

    Article Title: Tryptase Regulates the Epigenetic Modification of Core Histones in Mast Cell Leukemia Cells
    Article Snippet: Nafamostat mesylate (cat no N0289), staurosporine (cat no S4400), mefloquine (cat no M2319), Pefabloc SC (cat no 11429868001), pepstatin A (cat no 516481) and E-64d (cat no E8640) were from Sigma-Aldrich (Steinheim, Germany).

    Article Title: FAM114A1 Influences Cardiac Fibrosis by Regulating Angiotensin II Signaling in Cardiac Fibroblasts
    Article Snippet: Human angiotensin II (Cat. No. A9525), E-64d (Cat. No. E8640), 2,3 Butanedione Monoxime (Cat. No. B0753), and 5-Bromo-2’-deoxyuridine (Cat. No. 10280879001) were purchased from Sigma Aldrich, USA.

    Article Title: Enhanced Omicron subvariant cross-neutralization efficacy of a SARS-CoV-2 BA.4/5 mRNA vaccine encoding a noncleaved, nonfusogenic spike antigen
    Article Snippet: Camostat (cat. no. SML0057) and E-64d (cat. no. E8640) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Western Blot:

    Article Title: Supplementary Information for FAM114A1 Influences Cardiac Pathological Remodeling by Regulating Angiotensin II Signaling
    Article Snippet: Reagents, antibodies, plasmids, and siRNAs Human angiotensin II (Cat. No. A9525), E-64d (Cat. No. E8640), 2,3 Butanedione Monoxime (Cat. No. B0753), and 5-Bromo-2'-deoxyuridine (Cat. No. 10280879001) were purchased from Sigma Aldrich, USA.

    Article Title: Mixed-Charge Nanocarriers Allow for Selective Targeting of Mitochondria by Otherwise Nonselective Dyes.
    Article Snippet: Targeted delivery of molecular cargos to specific organelles is of paramount importance for developing precise and effective therapeutics and imaging probes.. This work describes a disulfide-based delivery method in which mixedcharged nanoparticles traveling through the endolysosomal tract deliver noncovalently bound dye molecules selectively into mitochondria.. This system comprises three elements: (1) The nanoparticles deliver their payloads by a kiss-and-go mechanism that is, they drop off their dye cargos proximate to mitochondria but do not localize therein; (2) the dye molecules are by themselves nonspecific to any cellular structures but become so with the help of mixed-charge nanocarriers; and (3) the dye is engineered in such a way as to remain in mitochondria for a long time, up to days, allowing for observing dynamic remodeling of mitochondrial networks and long-term tracking of mitochondria even in dividing cells.

    Article Title: Tryptase Regulates the Epigenetic Modification of Core Histones in Mast Cell Leukemia Cells
    Article Snippet: Nafamostat mesylate (cat no N0289), staurosporine (cat no S4400), mefloquine (cat no M2319), Pefabloc SC (cat no 11429868001), pepstatin A (cat no 516481) and E-64d (cat no E8640) were from Sigma-Aldrich (Steinheim, Germany).

    Article Title: FAM114A1 Influences Cardiac Fibrosis by Regulating Angiotensin II Signaling in Cardiac Fibroblasts
    Article Snippet: Human angiotensin II (Cat. No. A9525), E-64d (Cat. No. E8640), 2,3 Butanedione Monoxime (Cat. No. B0753), and 5-Bromo-2’-deoxyuridine (Cat. No. 10280879001) were purchased from Sigma Aldrich, USA.

    Article Title: Enhanced Omicron subvariant cross-neutralization efficacy of a SARS-CoV-2 BA.4/5 mRNA vaccine encoding a noncleaved, nonfusogenic spike antigen
    Article Snippet: Camostat (cat. no. SML0057) and E-64d (cat. no. E8640) were purchased from Sigma-Aldrich (St. Louis, MO, USA).



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    e-64d (cat no e8640)  (Millipore)
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    Treatment of mast cell leukemia cells with tryptase inhibitor abrogates H3 cleavage in response to cytotoxic agents. (A) HMC-1 cells (0.5 x 10 6 cells/ml) were incubated with protease inhibitors: Pefabloc SC (Pefa; 0.1 mM), nafamostat (Nafa; 20 μM), <t>E-64d</t> (15 μM), Pepstatin A (Pep A; 50 μM) or EDTA (20 μM) for 30 min prior to treating the cells with LLME (400 μM) for 24 h. Subsequently, samples corresponding to equal numbers of cells were subjected to Western blot analysis for histone 3 (H3), with ß-actin as loading control. (B) Quantification of the signal intensity for cleaved H3, as % of total H3. The presented data are representative of at least two independent experiments, and are given as mean values ± SEM (n=3). ****p ≤ 0.0001. ns, not significant.
    E 64d (Cat No E8640), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e-64d+(cat+no+e8640)/e64d/pmc08674432-121-24-30
    Average 90 stars, based on 1 article reviews
    e-64d (cat no e8640) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Treatment of mast cell leukemia cells with tryptase inhibitor abrogates H3 cleavage in response to cytotoxic agents. (A) HMC-1 cells (0.5 x 10 6 cells/ml) were incubated with protease inhibitors: Pefabloc SC (Pefa; 0.1 mM), nafamostat (Nafa; 20 μM), E-64d (15 μM), Pepstatin A (Pep A; 50 μM) or EDTA (20 μM) for 30 min prior to treating the cells with LLME (400 μM) for 24 h. Subsequently, samples corresponding to equal numbers of cells were subjected to Western blot analysis for histone 3 (H3), with ß-actin as loading control. (B) Quantification of the signal intensity for cleaved H3, as % of total H3. The presented data are representative of at least two independent experiments, and are given as mean values ± SEM (n=3). ****p ≤ 0.0001. ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: Tryptase Regulates the Epigenetic Modification of Core Histones in Mast Cell Leukemia Cells

    doi: 10.3389/fimmu.2021.804408

    Figure Lengend Snippet: Treatment of mast cell leukemia cells with tryptase inhibitor abrogates H3 cleavage in response to cytotoxic agents. (A) HMC-1 cells (0.5 x 10 6 cells/ml) were incubated with protease inhibitors: Pefabloc SC (Pefa; 0.1 mM), nafamostat (Nafa; 20 μM), E-64d (15 μM), Pepstatin A (Pep A; 50 μM) or EDTA (20 μM) for 30 min prior to treating the cells with LLME (400 μM) for 24 h. Subsequently, samples corresponding to equal numbers of cells were subjected to Western blot analysis for histone 3 (H3), with ß-actin as loading control. (B) Quantification of the signal intensity for cleaved H3, as % of total H3. The presented data are representative of at least two independent experiments, and are given as mean values ± SEM (n=3). ****p ≤ 0.0001. ns, not significant.

    Article Snippet: Nafamostat mesylate (cat no N0289), staurosporine (cat no S4400), mefloquine (cat no M2319), Pefabloc SC (cat no 11429868001), pepstatin A (cat no 516481) and E-64d (cat no E8640) were from Sigma-Aldrich (Steinheim, Germany).

    Techniques: Incubation, Western Blot